Ensuring the profitability of working biogas plants is a key factor for the development of biogas market. Apart from the price obtained for sold electricity, the substrates are the most important factor, particularly the ratio of their acquisition cost to methane efficiency. Thus, the objective of this paper was energy and economic analysis of the typical biogas plant with a capacity of 1 MWe working in new, favorable market situation (higher prices for blue certificates and in auction system) and using different biomass and waste substrates. After biogas efficiency tests and economic calculations of 4 different agricultural and waste substrates (maize silage, beet pulp, refood and chicken manure) it has been stated that the chicken manure was the most energy effective, just after maize silage. However chicken manure is 5 times cheaper. However, refood is the most profitable substrate for biogas plant working in both variants (certificates and auctions), and slightly less favorable - chicken manure. It is related to the best price - methane efficiency ratio, since the adoption of refood for biogas plants receives payment in the amount of 20 PLN per ton. In vast majority of analyzed substrates, investment in agricultural biogas plant is now becoming a very profitable venture, because annual profit before tax fluctuated in the range of 1-4 million PLN.