In the European Union, including Latvia there is an increasing interest in cultivation and production of industrial hemp (Cannabis sativa L.) fibres as well as seeds. The aim of the investigation was to evaluate N fertilization and sowing rate suitable for seed yield productivity of hemp cultivar ‘Purini’ in Latvian environmental conditions. Research was carried out to study the effect of the environmental factors in the cultivation years 2012 - 2013 and 2015, genotype (‘Purini’), nitrogen fertilizer doses (0, 30, 60 and 90 kg ∙ ha-1), seeding density (50, 70, 90 and 110 kg ∙ ha-1) in one vegetation period on hemp seed yield. In this case agronomically important traits, such as seed yield, 1000 seed mass and seed oil contents also were taken into account. Investigation has been conducted in the Agricultural Scientific Centre of Latgale in field trials. Yield parameters of hemp varied both by years and by treatments. Correlations showed statistically positive significant relationships between seed yield and nitrogen rate increasing in all years. Seed yield of hemp ranged from 0.83 to 2.39 t ∙ ha-1. In 2015 optimal plant seed density rate of 50 kg ∙ ha-1 by seed yield 2.05 t ∙ ha-1.