The rootstock is an important element in the production of fruit trees. Proper selection of rootstocks affects the quality of trees produced in the nursery and their growth and fruiting in the orchard. In 2015, an assessment was performed of the effect of the rootstock on the quality of apple trees produced in an organic nursery. The assessment and measurements were made in a nursery located in the Experimental Ecological Orchard of the Research Institute of Horticulture in Nowy Dwór Parcela near Skierniewice. The studied objects included seven-month-old trees of four apple cultivars: ‘Szampion’, ‘Gold Milenium’, ‘Topaz’ and ‘Pinova’, which had been grafted onto rootstocks of different growth vigour: M.9, M.26 and M.7. The highest number of trees in relation to the number of the grafted rootstocks planted was obtained for apple trees growing on the M.7 rootstock. The best quality trees of the tested apple cultivars were also obtained on the M.7 rootstock.