The quality of fruit trees obtained from a nursery is very important; it determines their precocity and productivity, and thus helps to obtain a better economic effect. In the years 2015-2016, the quality of two-year-old apple trees with a one-year-old crown (knip trees) was assessed in an organic nursery in relation to the rootstock used. The assessment and measurements were conducted in a nursery located in the Experimental Ecological Orchard of the Research Institute of Horticulture in Nowy Dwór Parcela near Skierniewice. The studied material consisted of two-year-old trees with one-year-old crown of the apple cultivars ‘Szampion’ and ‘Gold Milenium’, which were grafted onto rootstocks of different growth vigour: M.9, M.26 and M.7, in two thickness classes (8-10 mm and 10-12 mm). For both cultivars, the best quality of two-year-old trees with a one-year-old crown was obtained on the M.7 rootstock in both thickness classes.