Polycyclic aromatic hydrocarbons (PAHs) are a group of organic compounds, which accumulation in arable land is highly unfavorable phenomenon due to their toxic properties and adverse effects on yield as well as growth of plants. In order to determine the degree of PAHs exposure, the research on the sorption and accumulation of PAHs in various components of the soil is indispensable. The aim of this study was to define the effect of the organic and clay fraction on the mobility of PAHs. Experiments were carried out in column systems and the following solid phases (sorbents) were applied: a quartz sand (a control), a mixture of quartz sand and humic acids, a mixture of quartz sand and clay mineral - montmorillonite and systems combined with quartz sand, montmorillonite and humic acids. As a labile phase, the aqueous solution of phenanthrene (concentration of 0,5 mg•l-1) was used. Quantification of PAHs in the eluate was carried out by a photoluminescence method. The obtained results indicate that the increase of both clay fraction and the humic acids resulted in an increase in the sorption of phenanthrene on a solid matrix. Moreover, the montmorillonite showed higher sorption than humic acids added in the form of a commercial preparation. Thus, it can be assumed that the soils characterized by favorable agronomic properties (a lot of clay fraction and organic matter) are particularly susceptible to the accumulation of organic compounds (e.g. PAHs). Additionally, this kind of soil - due to immobilization - is characterized by smaller capacity of natural bioremediation. These conclusions points to the need for monitoring of arable land, particularly areas with high emissions of PAHs to the environment.