Progressing from the eighteenth century industrial development, transport and urban areas expansion contribute to the accumulation of polycyclic aromatic hydrocarbons (PAHs) in different components of the environment and their deposition into soils. Due to the established, highly adverse PAHs effects on living organisms, there is a tendency to develop effective remediation technologies which can result in PAHs removing from the environment. The aim of this study was to determine the effect of ozone-initial radical reactions on biodegradation kinetics of selected PAHs by an environmental consortium. The consortium was composed of microorganisms isolated from soil permanently contaminated with organic substances such as PAHs. The decomposition reactions were carried out under aerobic conditions at 25°C for 240h in model systems containing water and PAH (naphthalene, phenanthrene or pyrene) at a concentration of 50 mg/l. The ozonation process was conducted before microbial inoculation and lasted 60 minutes (yield 2-5 g of ozone/h). The final PAHs concentrations were measured by photochemiluminescent method. It was found that pre-ozonation does not result in the death of microorganisms. The biodegradation kinetics was evaluated by a comparison of PAH half-life degradation. The results indicate that radical reactions increase the biodegradation efficiency by 25%. These results demonstrate a good efficiency of combined techniques - ozonation and biodegradation - and can provide the possibility of their application in the protection of agricultural land against degradation.