This paper presents the results of the evaluation of feed quality conducted on the basis of the content of a tracer coated with a fluorescent substance. The study used the example of compound feed mixture for laying hens. Feed ingredients: maize and kardi primed wet using fluorescent solutions. The substances, such as Tinopal, Uranine and Rhodamine B were excited to shining by ultraviolet radiation. Before mixing process started, two tracers coated with different fluorescent substances were introduced into the mixture. The acquired images of the samples were analysed using the Patan computer program and were the basis of the information about the percentage share of the specified key component. The tests were used to verify the premise that there is a possibility of simultaneous determination of several components in a mixture based on fluorescent method. The results confirmed this assumption.